Whole genome sequencing (WGS) is important for study of life sciences, evolutionary biology and clinical progresses. The workshop started with the quality control of a sample sequence followed by trimming of adapters. Next, the sample was aligned with the reference genome and identifiers were added. Through the use of tools for SNP and indel callers, a Variant Calling Format file was generated, whose data was analysed and annotated by matching the SNPs against the NCBI database. The designed pipeline provided a holistic and realistic experience of how sequence analysis is used in the industry. The workshop was conducted by Ms. Shivangi Desai, Ms. Priyanka H.L. and Ms. S. Sneha from LeucineRichBio on Oct 17, 2019.